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KMID : 0364819870250010017
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Korean Journal of Microbiology
1987 Volume.25 No. 1 p.17 ~ p.22
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Characterization and biovar. Determination of Agrobacterium tumefaciens T7 isolated in Korea
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이윤/Lee, Y
김창진/김성훈/유익동/민태익/C.H. Kim/Kim, S.H./Yoo, I.D./Mheen, T.I.
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Abstract
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1
For the purpose of securing of strains which can be usefully utilized to study symbiosis between Rhizobium and legume plant, A. tumefaciens T7 was isolated and characterized and then subgroup biovar was determined. A. tumefaciens T7 induced smooth tumor like nopaline type one and did not grow at 37°C and in the presence of 2% NaCl on yeast extract mannitol medium. The strain was able to grow on the New and Kerr selective media and utilize erythritol but not phenylalanine, tryptophan, and tartarate as a sole carbon source. Negative results were obtained from 3-keto-lactose production and oxidase test. The strain produced alkali from malonate and citrate and showed acid litmus milk reaction. At least two large plasmids were detected in the cell lysate. According to all of these results, it could be concluded that subdivision of isolated strain was biovar 2.
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KEYWORD
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